pectronaut 21.0 is the Biognosys’ gold-standard software for data-independent acquisition (DIA) proteomics analysis. Trusted by researchers at leading institutions worldwide including Yale University, Max Planck Institute, and Bruker Corporation, it delivers unparalleled throughput and precision for protein identification and quantification across discovery, translational, and clinical research applications.

What’s New in Spectronaut 21.0

Advanced ML Scoring & AI Models

  • ~10% more protein groups identified on average across 65 proteomics datasets
  • ~11% more immunopeptides identified across 27 immunopeptidomics datasets
  • Next-generation AI models accurately predict unseen post-translational modifications (PTMs)
  • Improved AI predictions for both tryptic and unspecific peptides

Improved Computational Performance

  • Up to 30% faster merging steps when parallelizing
  • 10% faster directDIA for immunopeptidomics (Class I and II)
  • 15% faster directDIA for timsTOF on average
  • 50% smaller SNE file size when saving with XIC
  • New directDIA workflow that randomly sub-selects samples for library generation

Enhanced PTM Processes & Reporting

  • Improved PTM site localization for higher quality data
  • Option to collapse PTM site report by protein group
  • Further improvements in site stoichiometry calculations
  • New PTM site-specific statistics and visualizations in post-analysis

Simplified Analysis Settings

  • Simplified identification settings for directDIA with unchanged default behavior
  • Simplified mass tolerance settings for directDIA and library generation
  • New option to group peptides by In-Source Fragmentation events in tree view
  • Added summary for identifications per channel (e.g., SILAC experiment)
  • Option to automatically sub-sample runs during directDIA to increase analysis throughput