BioPharma Finder 5.4: Comprehensive Biotherapeutic Characterization Software

BioPharma Finder 5.4 is Thermo Fisher Scientific’s purpose-built software solution for the characterization of biotherapeutics using high-resolution accurate mass (HRAM) data from Orbitrap mass spectrometers. It streamlines data processing, curation, and reporting across multiple workflows in a connected environment .

Key Workflows and Features

Intact Protein Analysis

  • Molecular Weight Determination: Rapid measurement of intact molecular weight for structural confirmation and characterization

  • Confident Deconvolution: Superior algorithms for accurate deconvolution in both acidic and native conditions

  • Sequence Matching: Target protein sequence matching to identify N-linked glycosylations and common modifications

  • ADC Analysis: Antibody Drug Conjugate identification and Drug Antibody Ratio (DAR) determination using sliding window algorithm

  • Batch Comparison: Compare multiple batches side-by-side

Peptide Mapping

  • Sequence Validation: Novel MS2 prediction algorithm provides added confidence in amino acid sequence assignments

  • PTM Characterization: Identify site-specific expected and unknown post-translational modifications with relative quantitation

  • Disulfide Bond Mapping: Comprehensive analysis of disulfide linkages

  • Error-Tolerant Searches: Detect unexpected modifications and sequence alterations

  • De Novo Sequencing: Create searches for unknown components

  • Low-Level Detection: Identify impurities and sequence variants

Multi-Attribute Method (MAM)

  • CQA Monitoring: Characterize and quantify critical quality attributes using MS-based MAM

  • GMP-Ready Workflows: Seamless transition from discovery peptide mapping to GMP environment monitoring

  • Chromeleon Integration: Transfer workflows to Chromeleon CDS for compliant targeted quantitation and new peak detection

Top-Down Analysis

  • Simple Sequencing: Intact protein molecule sequencing using ProSightBP core algorithm

  • Multi-File Comparison: Compare multiple raw files with interactive fragmentation coverage maps

  • Multiple Fragmentation Modes: Support for CID, HCD, ETD, EThcD, and UVPD

BioPharma Finder 5.4
BioPharma Finder 5.4